Microscale Perfusion-based Cultivation for Pichia pastoris Clone

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redwoodbioscience

Microscale Perfusion-based Cultivation for Pichia pastoris Clone Screening Allows Accelerated and Optimized RecombinantProtein Manufacturing Processes

Pichia pastoris has emerged prior to now years as a promising host for recombinant protein and biopharmaceutical manufacturing. Contained in the institution of maximum cell density fed-batch biomanufacturing, screening half and early bioprocess progress (based mostly completely on microplates and shake flasks) nonetheless characterize a bottleneck attributable to high-cost and time-consuming procedures together with low experiment complexity.

Inside the current work, we implement a screening protocol developed for P. pastoris clone various in a multiplexed microfluidic machine with 15 μL cultivation chambers able to function in perfusion mode and monitor dissolved oxygen content material materials supplies all through the customized in a non-invasive means. The setup allowed us to determine carbon-limited circumstances and take into consideration stress responses to fully fully completely different enter variables. Outcomes from micro-scale perfusion cultures had been then in distinction with 1L fed-batch fermentation.

The best producer by way of titer and productiveness was shortly acknowledged after 12 hours from inoculation and the outcomes confirmed by lab-scale fermentation.

Furthermore, the physiological analyses of the strains beneath fully fully completely different circumstances steered how additional subtle experimental circumstances had been achievable regardless of the comparatively straightforward, straight-forward, and cost-effective experimental setup. Implementation and standardization of those micro-scale protocols may within the discount of the demand for lab-scale bioreactor cultivations thus accelerating the event of protein manufacturing processes. This textual content material is protected by copyright. All rights reserved.

redwoodbioscience
redwoodbioscience

Chicken Homeobox protein engrailed- 2, EN2 ELISA KIT

ELI-05953c 96 Tests
EUR 1113.6

Chicken Homeobox Protein Engrailed-2 (EN2) ELISA Kit

abx518615-96tests 96 tests
EUR 1093.2

Recombinant Engrailed Homeobox Protein 2 (EN2)

4-RPJ982Hu01
  • EUR 528.58
  • EUR 265.20
  • EUR 1652.16
  • EUR 630.72
  • EUR 1141.44
  • EUR 429.60
  • EUR 3950.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Engrailed Homeobox Protein 2 expressed in: E.coli

Homeobox Protein Engrailed-1 (EN1) Antibody

20-abx215150
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Homeobox Protein Engrailed-1 (EN1) Antibody

abx033297-400ul 400 ul
EUR 627.6

Homeobox Protein Engrailed-1 (EN1) Antibody

abx033297-80l 80 µl
EUR 343.2

Homeobox Protein Engrailed-1 (EN1) Antibody

20-abx300471
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Homeobox Protein Engrailed-1 (EN1) Antibody (HRP)

20-abx300472
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Homeobox Protein Engrailed-1 (EN1) Antibody (FITC)

20-abx300473
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin)

20-abx300474
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human Homeobox protein engrailed- 1, EN1 ELISA KIT

ELI-48646h 96 Tests
EUR 988.8

Mouse Homeobox protein engrailed- 1, En1 ELISA KIT

ELI-27118m 96 Tests
EUR 1038

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033410-400ul 400 ul
EUR 627.6

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033410-80l 80 µl
EUR 343.2

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033411-400ul 400 ul
EUR 627.6

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033411-80l 80 µl
EUR 343.2

Homeobox Protein Engrailed-2 (EN2) Antibody

20-abx302580
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Homeobox Protein Engrailed-2 (EN2) Antibody (HRP)

20-abx315705
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Engrailed Homeobox Protein 2 (EN2) Antibody

20-abx176276
  • EUR 1446.00
  • EUR 693.60
  • 1 mg
  • 200 ug

Engrailed Homeobox Protein 2 (EN2) Antibody

20-abx176277
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Engrailed Homeobox Protein 2 (EN2) Antibody

20-abx172243
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Homeobox Protein Engrailed-2 (EN2) Antibody (FITC)

20-abx315706
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human Homeobox protein engrailed- 2, EN2 ELISA KIT

ELI-05951h 96 Tests
EUR 988.8

Mouse Homeobox protein engrailed- 2, En2 ELISA KIT

ELI-05952m 96 Tests
EUR 1038

Human Engrailed Homeobox Protein 2 (EN2) Protein

20-abx652255
  • EUR 744.00
  • EUR 326.40
  • EUR 2230.80
  • EUR 878.40
  • EUR 543.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Homeobox Protein Engrailed-2 (EN2) Antibody (Biotin)

20-abx315707
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human Homeobox Protein Engrailed-2 (EN2) ELISA Kit

abx251286-96tests 96 tests
EUR 801.6

Mouse Homeobox Protein Engrailed-2 (EN2) ELISA Kit

abx518617-96tests 96 tests
EUR 801.6

Human Homeobox protein engrailed-2(EN2) ELISA kit

CSB-EL007660HU-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Homeobox protein engrailed-2 (EN2) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Homeobox protein engrailed-2(EN2) ELISA kit

1-CSB-EL007660HU
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Homeobox protein engrailed-2(EN2) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Homeobox protein engrailed-2(EN2) ELISA kit

EKC41536-48T 48T
EUR 535.99

Human Homeobox protein engrailed-2(EN2) ELISA kit

EKC41536-5x96T 5x96T
EUR 3637.08

Human Homeobox protein engrailed-2(EN2) ELISA kit

EKC41536-96T 96T
EUR 765.7

Mouse Homeobox protein engrailed-2 (EN2) ELISA Kit

RK08809 96T
EUR 280

Engrailed Homeobox 2 Protein (OVA)

20-abx165529
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human EN2/ Homeobox protein engrailed-2 ELISA Kit

E0787Hu 1 Kit
EUR 685.2

Human EN2(Homeobox protein engrailed-2) ELISA Kit

EH1964 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human EN2(Homeobox protein engrailed-2) ELISA Kit

EKF57407-48T 48T
EUR 396.9

Human EN2(Homeobox protein engrailed-2) ELISA Kit

EKF57407-5x96T 5x96T
EUR 2693.25

Human EN2(Homeobox protein engrailed-2) ELISA Kit

EKF57407-96T 96T
EUR 567

Engrailed Homeobox Protein 2 (EN2) Antibody Pair

20-abx370677
  • EUR 2097.60
  • EUR 1345.20
  • 10 × 96 tests
  • 5 × 96 tests

OVA conjugated Engrailed Homeobox Protein 2 (EN2)

4-CPJ982Hu21
  • EUR 496.32
  • EUR 256.80
  • EUR 1531.20
  • EUR 590.40
  • EUR 1060.80
  • EUR 408.00
  • EUR 3648.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Engrailed Homeobox Protein 2 expressed in: chemical synthesis

Human Engrailed Homeobox Protein 2 (EN2) CLIA Kit

20-abx495807
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

20-abx151405
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Engrailed Homeobox Protein 2 (EN2)ELISA Kit

201-12-2671 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

abx571248-96tests 96 tests
EUR 990

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

DLR-EN2-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates or other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

DLR-EN2-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates or other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

4-SEJ982Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Engrailed Homeobox Protein 2 ELISA Kit (EN2)

RK01311 96 Tests
EUR 625.2

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RDR-EN2-Hu-48Tests 48 Tests
EUR 652.8

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RDR-EN2-Hu-96Tests 96 Tests
EUR 907.2

Human Engrailed Homeobox Protein 2(EN2)ELISA Kit

QY-E04943 96T
EUR 433.2

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RD-EN2-Hu-48Tests 48 Tests
EUR 625.2

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RD-EN2-Hu-96Tests 96 Tests
EUR 867.6

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RDR-EN2-Hu-48T 48T
EUR 475.34
Description: tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RDR-EN2-Hu-96T 96T
EUR 679.04
Description: tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

DL-EN2-Hu 96T
EUR 441
Description: tissue homogenates or other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

DLR-EN2-Hu 96T
EUR 463
Description: tissue homogenates or other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2)ELISA Kit

YLA3312HU-48T 48T
EUR 435

Human Engrailed Homeobox Protein 2 (EN2)ELISA Kit

YLA3312HU-96T 96T
EUR 562.5

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RD-EN2-Hu-48T 48T
EUR 452.7
Description: tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RD-EN2-Hu-96T 96T
EUR 646.7
Description: tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKN45006-48T 48T
EUR 378.7

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKN45006-5x96T 5x96T
EUR 2569.75

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKN45006-96T 96T
EUR 541

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKU03895-48T 48T
EUR 555.66

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKU03895-5x96T 5x96T
EUR 3770.55

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKU03895-96T 96T
EUR 793.8

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKL57008-5x96T 5x96T
EUR 3793.35

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKL57008-96T 96T
EUR 798.6

ELISA kit for Human EN2 (Engrailed Homeobox Protein 2)

ELK3841 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Engrailed Homeobox Protein 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Rat Engrailed Homeobox Protein 2 ELISA kit

E02E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Engrailed Homeobox Protein 2 ELISA kit

E02E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Engrailed Homeobox Protein 2 ELISA kit

E02E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Engrailed Homeobox Protein 2 ELISA kit

E08E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Engrailed Homeobox Protein 2 ELISA kit

E08E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Engrailed Homeobox Protein 2 ELISA kit

E08E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Engrailed Homeobox Protein 2 ELISA kit

E07E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Engrailed Homeobox Protein 2 ELISA kit

E07E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Engrailed Homeobox Protein 2 ELISA kit

E07E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Engrailed Homeobox Protein 2 ELISA kit

E06E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Engrailed Homeobox Protein 2 ELISA kit

E06E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Engrailed Homeobox Protein 2 ELISA kit

E06E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Engrailed Homeobox Protein 2 ELISA kit

E03E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Engrailed Homeobox Protein 2 ELISA kit

E03E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Engrailed Homeobox Protein 2 ELISA kit

E03E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Engrailed Homeobox Protein 2 ELISA kit

E01E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Engrailed Homeobox Protein 2 ELISA kit

E01E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Engrailed Homeobox Protein 2 ELISA kit

E01E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Engrailed Homeobox Protein 2 ELISA Kit

IHUEN2KT each
EUR 733
Description: Human Engrailed Homeobox Protein 2 ELISA Kit

ELISA kit for Human Homeobox protein engrailed-2

EK4019 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Homeobox protein engrailed-2 in samples from serum, plasma, tissue homogenates and other biological fluids.

Rabbit Engrailed Homeobox Protein 2 ELISA kit

E04E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Engrailed Homeobox Protein 2 ELISA kit

E04E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Engrailed Homeobox Protein 2 ELISA kit

E04E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Engrailed Homeobox Protein 2 ELISA kit

E09E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Engrailed Homeobox Protein 2 ELISA kit

E09E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Engrailed Homeobox Protein 2 ELISA kit

E09E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Engrailed Homeobox Protein 2 (EN2) Magnetic Luminex Assay Kit

LKU601526-96T 96T
EUR 966

Guinea pig Engrailed Homeobox Protein 2 ELISA kit

E05E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Engrailed Homeobox Protein 2 ELISA kit

E05E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Engrailed Homeobox Protein 2 ELISA kit

E05E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant Human EN1/Engrailed1 Protein, His tag

E40KMH608 20ug
EUR 495

Engrailed 1 Antibody / EN1

F54331-0.05ML 0.05 ml
EUR 140.25
Description: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Engrailed 1 Antibody / EN1

F54331-0.2ML 0.2 ml
EUR 330.65
Description: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

EN1 (Engrailed 1) (N-term) Rabbit pAb

E2616140 100ul
EUR 225
Description: Available in various conjugation types.

Anti-EN1 / Engrailed 1 (3H2)

YF-MA10275 100 ug
EUR 435.6
Description: Mouse monoclonal to EN1 / Engrailed 1

Anti-EN1 / Engrailed 1 (1F5)

YF-MA10276 100 ug
EUR 435.6
Description: Mouse monoclonal to EN1 / Engrailed 1

Anti-EN1 / Engrailed 1 (3E5)

YF-MA12843 100 ug
EUR 435.6
Description: Mouse monoclonal to EN1 / Engrailed 1

Anti-EN1 / Engrailed 1 (3G9)

YF-MA12844 100 ug
EUR 435.6
Description: Mouse monoclonal to EN1 / Engrailed 1

Polyclonal EN1 (Engrailed 1) Antibody (N-term)

APR11818G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EN1 (Engrailed 1) (N-term). This antibody is tested and proven to work in the following applications:

Monoclonal EN1 / Engrailed Antibody (clone 1F5), Clone: 1F5

APR11819G 0.05mg
EUR 633.6
Description: A Monoclonal antibody against Human EN1 / Engrailed (clone 1F5). The antibodies are raised in Mouse and are from clone 1F5. This antibody is applicable in WB and IHC-P, E

Monoclonal EN1 / Engrailed Antibody (clone 3H2), Clone: 3H2

APR11820G 0.05mg
EUR 633.6
Description: A Monoclonal antibody against Human EN1 / Engrailed (clone 3H2). The antibodies are raised in Mouse and are from clone 3H2. This antibody is applicable in WB and IHC-P, E

Chicken Homeobox protein CDX- 1, CDX1 ELISA KIT

ELI-50558c 96 Tests
EUR 1113.6

Chicken Homeobox protein SAX- 1, SAX1 ELISA KIT

ELI-30504c 96 Tests
EUR 1113.6

Chicken Homeobox protein MSX- 1, MSX1 ELISA KIT

ELI-42082c 96 Tests
EUR 1113.6

Chicken Homeobox protein HMX3, HMX3 ELISA KIT

ELI-48669c 96 Tests
EUR 1113.6

Chicken Homeobox protein HMX1, HMX1 ELISA KIT

ELI-48111c 96 Tests
EUR 1113.6

Chicken Homeobox protein SIX6, SIX6 ELISA KIT

ELI-29827c 96 Tests
EUR 1113.6

Chicken Homeobox protein DBX2, DBX2 ELISA KIT

ELI-32126c 96 Tests
EUR 1113.6

Chicken Homeobox protein SIX3, SIX3 ELISA KIT

ELI-52983c 96 Tests
EUR 1113.6

Chicken Homeobox protein BarH- like 1, BARX1 ELISA KIT

ELI-49945c 96 Tests
EUR 1113.6

Chicken Homeobox protein DLX- 5, DLX5 ELISA KIT

ELI-08285c 96 Tests
EUR 1113.6

Chicken Homeobox protein GBX- 2, GBX2 ELISA KIT

ELI-31259c 96 Tests
EUR 1113.6

Chicken Homeobox protein MSX- 2, MSX2 ELISA KIT

ELI-16603c 96 Tests
EUR 1113.6

Chicken Homeobox protein goosecoid, GSC ELISA KIT

ELI-42876c 96 Tests
EUR 1113.6

Chicken Prospero homeobox protein 1, PROX1 ELISA KIT

ELI-36234c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A5, HOXA5 ELISA KIT

ELI-37575c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D1, HOXD1 ELISA KIT

ELI-37581c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- B3, HOXB3 ELISA KIT

ELI-48719c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D9, HOXD9 ELISA KIT

ELI-48280c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A4, HOXA4 ELISA KIT

ELI-07943c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A9, HOXA9 ELISA KIT

ELI-42169c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A2, HOXA2 ELISA KIT

ELI-30995c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- B8, HOXB8 ELISA KIT

ELI-30999c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- C8, HOXC8 ELISA KIT

ELI-31002c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D8, HOXD8 ELISA KIT

ELI-31005c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- B5, HOXB5 ELISA KIT

ELI-43341c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- B1, HOXB1 ELISA KIT

ELI-20229c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D4, HOXD4 ELISA KIT

ELI-20235c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A3, HOXA3 ELISA KIT

ELI-20550c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A7, HOXA7 ELISA KIT

ELI-20551c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- B6, HOXB6 ELISA KIT

ELI-20552c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A6, HOXA6 ELISA KIT

ELI-20856c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- B4, HOXB4 ELISA KIT

ELI-20861c 96 Tests
EUR 1113.6

MSX1 ELISA Kit| chicken Homeobox protein MSX-1 ELISA Kit

EF012401 96 Tests
EUR 826.8

Chicken Homeobox protein Hox- A11, HOXA11 ELISA KIT

ELI-07942c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A13, HOXA13 ELISA KIT

ELI-08395c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D13, HOXD13 ELISA KIT

ELI-31004c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D10, HOXD10 ELISA KIT

ELI-43343c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D12, HOXD12 ELISA KIT

ELI-14318c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D11, HOXD11 ELISA KIT

ELI-20554c 96 Tests
EUR 1113.6

Chicken Homeobox protein Nkx- 2.5, NKX-2.5 ELISA KIT

ELI-23180c 96 Tests
EUR 1113.6

Chicken Homeobox protein CHOX- 7, CHOX-7 ELISA KIT

ELI-48108c 96 Tests
EUR 1113.6

Chicken Homeobox protein GHOX- 7, GHOX-7 ELISA KIT

ELI-42131c 96 Tests
EUR 1113.6

Engrailed 1 Rabbit mAb

A19672 100μL
EUR 128.7
Description: A synthetic peptide corresponding to a sequence within amino acids 70-150 of human UBE2C (O00762).

A protein fragment of Rv3194c positioned on mycobacterial cell flooring efficiently prevents adhesion of recombinant Mycobacterium smegmatis, and ensures a mannequin new anti-adhesive drug

Adhesins are virulence parts expressed on the surfaces of pathogenic micro organism that mediate pathogen-host interactions, a significant step all through the an an an infection course of. Correct proper right here, we present that the Mycobacterium tuberculosis protease Rv3194c capabilities not solely as an enzyme however as an adhesin. The heterologous Rv3194c protein was purified from Escherichia coli and was confirmed to bind to hyaluronic acid (HA).

The HA-binding web site was acknowledged as a 20 amino acid peptide between residues 91 and 110 (P91-110). Rv3194c sure to A549 alveolar basal epithelial cells and the interplay was abolished by the addition of hyaluronidase or P91-110. Experimental an an an infection in vitro revealed that Rv3194c participates all through the attachment of recombinant Mycobacterium smegmatis (Rv3194c/MS) to A549 cells, and P91-110 remedy of A549 cells largely inhibited the Rv3194c/MS-A549 cell interplay.

To produce in vivo proof, we constructed a reporter stress of M. smegmatis that expressed a by-product of the firefly luciferase that’s shifted to pink (FFlucRT) together with Rv3194c (Rv3194c + FFlucRT/MS) to contaminate mice and monitor the occasion of the illness. In mice, Rv3194c dramatically enhanced M. smegmatis persistence and induced lesions all through the lungs. Along with, remedy of intratracheal Rv3194c + FFlucRT/MS- contaminated mice with P91-110 considerably suppressed the expansion of Rv3194c + FFlucRT/MS in vivo and diminished pathological harm launched on by an an an infection of the lung with Rv3194c + FFlucRT/MS.

Taken collectively, these outcomes reveal that Rv3194c capabilities as an HA-binding adhesin and that P91-110 may have the potential for treating and stopping mycobacterial an an an infection.

Lysine acylation utilizing conjugating enzymes for site-specific modification and ubiquitination of recombinantproteins

Enzymes are extraordinarily environment friendly units for protein labelling attributable to their specificity and delicate response circumstances. Many protocols, nonetheless, are restricted to modifications at protein termini, rely on non-peptidic metabolites or require giant recognition domains. Correct proper right here we report a chemoenzymatic methodology, which we title lysine acylation utilizing conjugating enzymes (LACE), to site-specifically modify folded proteins at inside lysine residues. LACE relies upon upon a minimal genetically encoded tag (4 residues) acknowledged by the E2 small ubiquitin-like modifier-conjugating enzyme Ubc9, and peptide or protein thioesters.

Collectively, this technique obviates the necessity for E1 and E3 enzymes, enabling isopeptide formation with merely Ubc9 in a programmable methodology. We reveal the utility of LACE by the site-specific attachment of biochemical probes, one-pot dual-labelling collectively with sortase, and the conjugation of wild-type ubiquitin and ISG15 to recombinant proteins.

Responses of leaf gas alternate attributes, photosynthetic pigments and antioxidant enzymes in NaCl-stressed cotton (Gossypiumhirsutum L.) seedlings to exogenous glycine betaine and salicylic acid

Background: Utility of exogenous glycine betaine (GB) and exogenous salicylic acid (SA) mitigates the hostile outcomes of salinity. Foliar spraying with exogenous GB or SA alleviates salt stress in vegetation by rising leaf gas alternate and stimulating antioxidant enzyme train. The results of foliar utility of exogenous GB and SA on the physiology and biochemistry of cotton seedlings subjected to salt stress keep unclear.

Outcomes: Outcomes confirmed that salt stress of 150 mMNaCl significantly diminished leaf gas alternate and chlorophyll fluorescence and decreased photosynthetic pigment parts and leaf relative water content material materials.

Foliar spray concentrations of 5.Zero mM exogenous GB and 1.Zero mM exogenous SA promoted gas alternate and fluorescence in cotton seedlings, elevated parts of chlorophyll pigments, and stimulated the antioxidant enzyme train. The foliar spray moreover elevated leaf relative water content material materials and endogenous GB and SA content material materials in contrast with the salt-stressed solely administration.

Whatever the salt-induced improve in antioxidant enzyme content material materials, exogenous GB and SA in experimental concentrations significantly elevated the train of glutathione reductase, ascorbate peroxidase, superoxide dismutase, catalase and peroxidase, and decreased malondialdehyde content material materials beneath salt stress.

All through all experimental foliar spray GB and SA concentrations, the photochemical effectivity of photosystem II (FV/FM) reached a peak at a spotlight of 5.Zero mM GB. The net photosynthetic worth (Pn) and FV/FM had been positively correlated with chlorophyll a and chlorophyll b content material materials in response to foliar spraying of exogenous GB and SA beneath salt stress.

 

Conclusions: We concluded, from our outcomes, that concentrations of 5.Zero mM GB or 1.Zero mM SA are optimum selections for mitigating NaCl-induced hurt in cotton seedlings on account of they promote leaf photosynthesis, improve parts of photosynthetic pigments, and stimulate antioxidant enzyme train. Amongst, 5.Zero mM GB and 1.Zero mM SA, the easiest effectivity in enhancing endogenous GB and SA concentrations was obtained with the foliar utility of 1.Zero mM SA beneath salt stress.

 

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