Microscale Perfusion-based Cultivation for Pichia pastoris Clone

0 Comments
redwoodbioscience

Microscale Perfusion-based Cultivation for Pichia pastoris Clone Screening Allows Accelerated and Optimized RecombinantProtein Manufacturing Processes

Pichia pastoris has emerged prior to now years as a promising host for recombinant protein and biopharmaceutical manufacturing. Contained in the institution of maximum cell density fed-batch biomanufacturing, screening half and early bioprocess progress (based mostly completely on microplates and shake flasks) nonetheless characterize a bottleneck attributable to high-cost and time-consuming procedures together with low experiment complexity.

Inside the current work, we implement a screening protocol developed for P. pastoris clone various in a multiplexed microfluidic machine with 15 μL cultivation chambers able to function in perfusion mode and monitor dissolved oxygen content material materials supplies all through the customized in a non-invasive means. The setup allowed us to determine carbon-limited circumstances and take into consideration stress responses to fully fully completely different enter variables. Outcomes from micro-scale perfusion cultures had been then in distinction with 1L fed-batch fermentation.

The best producer by way of titer and productiveness was shortly acknowledged after 12 hours from inoculation and the outcomes confirmed by lab-scale fermentation.

Furthermore, the physiological analyses of the strains beneath fully fully completely different circumstances steered how additional subtle experimental circumstances had been achievable regardless of the comparatively straightforward, straight-forward, and cost-effective experimental setup. Implementation and standardization of those micro-scale protocols may within the discount of the demand for lab-scale bioreactor cultivations thus accelerating the event of protein manufacturing processes. This textual content material is protected by copyright. All rights reserved.

redwoodbioscience
redwoodbioscience

Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin)

20-abx300474
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human Homeobox protein engrailed- 1, EN1 ELISA KIT

ELI-48646h 96 Tests
EUR 988.8

Mouse Homeobox protein engrailed- 1, En1 ELISA KIT

ELI-27118m 96 Tests
EUR 1038

Anti-EN1 / Engrailed 1 (3H2)

YF-MA10275 100 ug
EUR 435.6
Description: Mouse monoclonal to EN1 / Engrailed 1

Anti-EN1 / Engrailed 1 (1F5)

YF-MA10276 100 ug
EUR 435.6
Description: Mouse monoclonal to EN1 / Engrailed 1

Anti-EN1 / Engrailed 1 (3E5)

YF-MA12843 100 ug
EUR 435.6
Description: Mouse monoclonal to EN1 / Engrailed 1

Anti-EN1 / Engrailed 1 (3G9)

YF-MA12844 100 ug
EUR 435.6
Description: Mouse monoclonal to EN1 / Engrailed 1

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-100ug

QP5983-ec-100ug 100ug
EUR 848.4

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-10ug

QP5983-ec-10ug 10ug
EUR 391.2

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-1mg

QP5983-ec-1mg 1mg
EUR 2763.6

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-200ug

QP5983-ec-200ug 200ug
EUR 1338

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-500ug

QP5983-ec-500ug 500ug
EUR 1816.8

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-50ug

QP5983-ec-50ug 50ug
EUR 522

Chicken Homeobox Protein Engrailed-2 (EN2) ELISA Kit

abx518615-96tests 96 tests
EUR 1093.2

Chicken Homeobox protein engrailed- 2, EN2 ELISA KIT

ELI-05953c 96 Tests
EUR 1113.6

Polyclonal EN1 (Engrailed 1) Antibody (N-term)

APR11818G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EN1 (Engrailed 1) (N-term). This antibody is tested and proven to work in the following applications:

Recombinant Engrailed Homeobox Protein 2 (EN2)

4-RPJ982Hu01
  • EUR 528.58
  • EUR 265.20
  • EUR 1652.16
  • EUR 630.72
  • EUR 1141.44
  • EUR 429.60
  • EUR 3950.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Engrailed Homeobox Protein 2 expressed in: E.coli

Engrailed Homeobox 2 Protein (OVA)

20-abx165529
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Engrailed Homeobox Protein 2 (EN2) Antibody

20-abx172243
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033410-400ul 400 ul
EUR 627.6

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033410-80l 80 µl
EUR 343.2

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033411-400ul 400 ul
EUR 627.6

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033411-80l 80 µl
EUR 343.2

Homeobox Protein Engrailed-2 (EN2) Antibody

20-abx302580
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Engrailed Homeobox Protein 2 (EN2) Antibody

20-abx176276
  • EUR 1446.00
  • EUR 693.60
  • 1 mg
  • 200 ug

Engrailed Homeobox Protein 2 (EN2) Antibody

20-abx176277
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Monoclonal EN1 / Engrailed Antibody (clone 1F5), Clone: 1F5

APR11819G 0.05mg
EUR 633.6
Description: A Monoclonal antibody against Human EN1 / Engrailed (clone 1F5). The antibodies are raised in Mouse and are from clone 1F5. This antibody is applicable in WB and IHC-P, E

Monoclonal EN1 / Engrailed Antibody (clone 3H2), Clone: 3H2

APR11820G 0.05mg
EUR 633.6
Description: A Monoclonal antibody against Human EN1 / Engrailed (clone 3H2). The antibodies are raised in Mouse and are from clone 3H2. This antibody is applicable in WB and IHC-P, E

Human Homeobox protein engrailed-2(EN2) ELISA kit

1-CSB-EL007660HU
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Homeobox protein engrailed-2(EN2) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Engrailed Homeobox Protein 2 (EN2) Protein

20-abx652255
  • EUR 744.00
  • EUR 326.40
  • EUR 2230.80
  • EUR 878.40
  • EUR 543.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

OVA conjugated Engrailed Homeobox Protein 2 (EN2)

4-CPJ982Hu21
  • EUR 496.32
  • EUR 256.80
  • EUR 1531.20
  • EUR 590.40
  • EUR 1060.80
  • EUR 408.00
  • EUR 3648.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Engrailed Homeobox Protein 2 expressed in: chemical synthesis

Human Engrailed Homeobox Protein 2 ELISA kit

E01E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Engrailed Homeobox Protein 2 ELISA kit

E01E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Engrailed Homeobox Protein 2 ELISA kit

E01E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Engrailed Homeobox Protein 2 ELISA kit

E06E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Engrailed Homeobox Protein 2 ELISA kit

E06E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Engrailed Homeobox Protein 2 ELISA kit

E06E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Engrailed Homeobox Protein 2 ELISA kit

E02E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Engrailed Homeobox Protein 2 ELISA kit

E02E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Engrailed Homeobox Protein 2 ELISA kit

E02E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Engrailed Homeobox Protein 2 ELISA kit

E04E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Engrailed Homeobox Protein 2 ELISA kit

E04E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Engrailed Homeobox Protein 2 ELISA kit

E04E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Engrailed Homeobox Protein 2 ELISA kit

E08E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Engrailed Homeobox Protein 2 ELISA kit

E08E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Engrailed Homeobox Protein 2 ELISA kit

E08E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Engrailed Homeobox Protein 2 ELISA kit

E03E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Engrailed Homeobox Protein 2 ELISA kit

E03E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Engrailed Homeobox Protein 2 ELISA kit

E03E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Engrailed Homeobox Protein 2 ELISA kit

E07E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Engrailed Homeobox Protein 2 ELISA kit

E07E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Engrailed Homeobox Protein 2 ELISA kit

E07E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Engrailed Homeobox Protein 2 (EN2) Antibody Pair

20-abx370677
  • EUR 2097.60
  • EUR 1345.20
  • 10 × 96 tests
  • 5 × 96 tests

Homeobox Protein Engrailed-2 (EN2) Antibody (HRP)

20-abx315705
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Homeobox Protein Engrailed-2 (EN2) Antibody (FITC)

20-abx315706
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Homeobox Protein Engrailed-2 (EN2) Antibody (Biotin)

20-abx315707
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Monkey Engrailed Homeobox Protein 2 ELISA kit

E09E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Engrailed Homeobox Protein 2 ELISA kit

E09E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Engrailed Homeobox Protein 2 ELISA kit

E09E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

DLR-EN2-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates or other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

DLR-EN2-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates or other biological fluids.

Human Homeobox protein engrailed-2(EN2) ELISA kit

CSB-EL007660HU-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Homeobox protein engrailed-2 (EN2) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Guinea pig Engrailed Homeobox Protein 2 ELISA kit

E05E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Engrailed Homeobox Protein 2 ELISA kit

E05E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Engrailed Homeobox Protein 2 ELISA kit

E05E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human EN2/ Homeobox protein engrailed-2 ELISA Kit

E0787Hu 1 Kit
EUR 685.2

ELISA kit for Human Homeobox protein engrailed-2

EK4019 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Homeobox protein engrailed-2 in samples from serum, plasma, tissue homogenates and other biological fluids.

Human EN2(Homeobox protein engrailed-2) ELISA Kit

EH1964 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human Engrailed Homeobox Protein 2 (EN2)ELISA Kit

201-12-2671 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

20-abx151405
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Engrailed Homeobox Protein 2 (EN2) CLIA Kit

20-abx495807
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

abx571248-96tests 96 tests
EUR 990

Mouse Homeobox Protein Engrailed-2 (EN2) ELISA Kit

abx518617-96tests 96 tests
EUR 801.6

Human Homeobox Protein Engrailed-2 (EN2) ELISA Kit

abx251286-96tests 96 tests
EUR 801.6

Human Homeobox protein engrailed- 2, EN2 ELISA KIT

ELI-05951h 96 Tests
EUR 988.8

Mouse Homeobox protein engrailed- 2, En2 ELISA KIT

ELI-05952m 96 Tests
EUR 1038

Human Engrailed Homeobox Protein 2(EN2)ELISA Kit

QY-E04943 96T
EUR 433.2

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RD-EN2-Hu-48Tests 48 Tests
EUR 625.2

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RD-EN2-Hu-96Tests 96 Tests
EUR 867.6

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RDR-EN2-Hu-48Tests 48 Tests
EUR 652.8

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RDR-EN2-Hu-96Tests 96 Tests
EUR 907.2

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

4-SEJ982Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Engrailed Homeobox Protein 2 ELISA Kit (EN2)

RK01311 96 Tests
EUR 625.2

EN1 Recombinant Protein (Mouse)

RP131690 100 ug Ask for price

EN1 Recombinant Protein (Human)

RP056658 100 ug Ask for price

EN1 Antibody

1-CSB-PA007659LA01CH
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EN1. Recognizes EN1 from Chicken. This antibody is Unconjugated. Tested in the following application: ELISA

ELISA kit for Human EN2 (Engrailed Homeobox Protein 2)

ELK3841 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Engrailed Homeobox Protein 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Chicken CD40LG Recombinant Protein

R01114-1 5ug/vial
EUR 310.8
Description: CD40 Ligand (CD40LG) is a transmembrane protein that is a member of the TNF superfamily. In addition to existing in a membrane bound form, CD40LG can also be released as a soluble molecule (sCD40LG), which has been shown to have biological functions.CD40 interacts with CD40 ligand (aka CD40LG, TNFSF5 or CD154), which is predominantly expressed on activated CD4+ T-cells. Chicken CD40 Ligand Recombinant Protein is purified CD40LG produced in yeast.

Chicken IL-10 Recombinant Protein

R00021-1 5ug/vial
EUR 310.8
Description: IL-10 is an anti-inflammatory cytokine and a member of the IL-10 family of cytokines, which have indispensable functions in many infectious and inflammatory diseases. Chicken IL-10 Recombinant Protein is purified interleukin-10 produced in yeast.

Chicken IL-18 Recombinant Protein

R00124-1 5ug/vial
EUR 310.8
Description: Interleukin-18 (IL-18) is a proinflammatory cytokine that belongs to the IL-1 superfamily. IL-18 works together with IL-12 to induce cell-mediated immunity following infection, and IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders. Chicken IL-18 Recombinant Protein is purified interleukin-18 produced in yeast.

Chicken IL-4 Recombinant Protein

R00230-1 5ug/vial
EUR 310.8
Description: IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. Chicken IL-4 Recombinant Protein is purified interleukin-4 produced in yeast.

Chicken IL-16 Recombinant Protein

R01635-1 5ug/vial
EUR 310.8
Description: Interleukin 16 (IL-16) is a pleiotropic cytokine that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4, and it has effects on mixed lymphocyte reaction and inhibition of HIV viral replication. Chicken IL-16 Recombinant Protein is purified interleukin-16 produced in yeast.

Chicken IL-17F Recombinant Protein

R02062-1 5ug/vial
EUR 310.8
Description: IL-17F induces the production of many other cytokines, chemokines, and prostaglandins from many cell types (fibroblasts, endothelial cells, epithelial cells, keratinocytes and macrophages). IL-17F has been shown to have a pro-inflammatory role in asthma. Chicken IL-17F Recombinant Protein is purified interleukin-17F produced in yeast.

Chicken Homeobox protein MSX- 1, MSX1 ELISA KIT

ELI-42082c 96 Tests
EUR 1113.6

Chicken Homeobox protein SAX- 1, SAX1 ELISA KIT

ELI-30504c 96 Tests
EUR 1113.6

Chicken Homeobox protein CDX- 1, CDX1 ELISA KIT

ELI-50558c 96 Tests
EUR 1113.6

Chicken Prospero homeobox protein 1, PROX1 ELISA KIT

ELI-36234c 96 Tests
EUR 1113.6

EN1 Antibody

DF8055 200ul
EUR 420

EN1 antibody

70R-3473 50 ug
EUR 560.4
Description: Rabbit polyclonal EN1 antibody raised against the C terminal of EN1

EN1 Antibody

47107-100ul 100ul
EUR 302.4

EN1 Antibody

45204-100ul 100ul
EUR 302.4

EN1 Antibody

45204-50ul 50ul
EUR 224.4

EN1 Antibody

ABD8055 100 ug
EUR 525.6

EN1 siRNA

20-abx915383
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

EN1 siRNA

20-abx915384
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

Chicken IL-12 p40 Recombinant Protein

R01152-1 5ug/vial
EUR 310.8
Description: The IL-12 p40 subunit, or interleukin-12 beta subunit, is the 40 kD cytokine receptor-like subunit of the heterodimeric cytokines IL-12 and IL-23. IL-12 is involved in the differentiation of naive T cells into Th1 cells and the growth and function of T cells. Both IL-23 and IL-12 can activate the transcription activator STAT4 and stimulate the production of interferon-gamma (IFNG). Chicken IL-12 p40 Recombinant Protein is purified interleukin-12 p40 produced in yeast.

Chicken Pituitary homeobox 1, PITX1 ELISA KIT

ELI-45279c 96 Tests
EUR 1113.6

EN1 Antibody, HRP conjugated

1-CSB-PA007659LB01CH
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EN1. Recognizes EN1 from Chicken. This antibody is HRP conjugated. Tested in the following application: ELISA

EN1 Antibody, FITC conjugated

1-CSB-PA007659LC01CH
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EN1. Recognizes EN1 from Chicken. This antibody is FITC conjugated. Tested in the following application: ELISA

EN1 Antibody, Biotin conjugated

1-CSB-PA007659LD01CH
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EN1. Recognizes EN1 from Chicken. This antibody is Biotin conjugated. Tested in the following application: ELISA

Anti-Homeobox protein SIX6 Antibody

A06502-1 100ug/vial
EUR 352.8

Chicken Homeobox protein BarH- like 1, BARX1 ELISA KIT

ELI-49945c 96 Tests
EUR 1113.6

Chicken Paired mesoderm homeobox protein 1, PRRX1 ELISA KIT

ELI-16801c 96 Tests
EUR 1113.6

POU5F1 Human, PolyR POU Class 5 Homeobox 1 Human Recombinant Protein, Polyarginine-Tag

PROTQ01860-1 Regular: 10ug
EUR 380.4
Description: POU5F1 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 360 amino acids and having a molecular mass of 38.6kDa.;The POU5F1 is fused to a C-terminal poly-arginine tag and purified by proprietary chromatographic techniques.

Chicken Homeobox protein goosecoid, GSC ELISA KIT

ELI-42876c 96 Tests
EUR 1113.6

Chicken Homeobox protein DBX2, DBX2 ELISA KIT

ELI-32126c 96 Tests
EUR 1113.6

Chicken Homeobox protein SIX3, SIX3 ELISA KIT

ELI-52983c 96 Tests
EUR 1113.6

Chicken Homeobox protein SIX6, SIX6 ELISA KIT

ELI-29827c 96 Tests
EUR 1113.6

Chicken Homeobox protein HMX1, HMX1 ELISA KIT

ELI-48111c 96 Tests
EUR 1113.6

Chicken Homeobox protein HMX3, HMX3 ELISA KIT

ELI-48669c 96 Tests
EUR 1113.6

EN1 Blocking Peptide

DF8055-BP 1mg
EUR 234

EN1 Conjugated Antibody

C45204 100ul
EUR 476.4

EN1 Conjugated Antibody

C47107 100ul
EUR 476.4

EN1 Blocking Peptide

33R-5203 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of EN1 antibody, catalog no. 70R-3473

Chicken Ventral anterior homeobox 1, VAX1 ELISA KIT

ELI-44665c 96 Tests
EUR 1113.6

Chicken Visual system homeobox 1, VSX1 ELISA KIT

ELI-40540c 96 Tests
EUR 1113.6

Chicken T- cell leukemia homeobox protein 1, TLX1 ELISA KIT

ELI-41860c 96 Tests
EUR 1113.6

MSX1 ELISA Kit| chicken Homeobox protein MSX-1 ELISA Kit

EF012401 96 Tests
EUR 826.8

PRRX1 ELISA Kit| chicken Paired mesoderm homeobox protein 1 ELI

EF012447 96 Tests
EUR 826.8

PROX1 ELISA Kit| chicken Prospero homeobox protein 1 ELISA Kit

EF012473 96 Tests
EUR 826.8

Engrailed-2 Peptide

45-537P 0.1 mg
EUR 405.6
Description: Engrailed-2 Peptide

Engrailed-2 Antibody

abx432651-200ul 200 ul
EUR 460.8

Recombinant NK6 Homeobox Protein 1 (NKX6-1)

4-RPG781Hu01
  • EUR 612.44
  • EUR 286.80
  • EUR 1966.66
  • EUR 735.55
  • EUR 1351.10
  • EUR 484.80
  • EUR 4736.64
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human NK6 Homeobox Protein 1 expressed in: E.coli

Chicken LIM/homeobox protein Lhx9, LHX9 ELISA KIT

ELI-42156c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A9, HOXA9 ELISA KIT

ELI-42169c 96 Tests
EUR 1113.6

Chicken Retinal homeobox protein Rx2, RX2 ELISA KIT

ELI-42251c 96 Tests
EUR 1113.6

Chicken LIM/homeobox protein Lhx1, LHX1 ELISA KIT

ELI-43192c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- B5, HOXB5 ELISA KIT

ELI-43341c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D10, HOXD10 ELISA KIT

ELI-43343c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A2, HOXA2 ELISA KIT

ELI-30995c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- B8, HOXB8 ELISA KIT

ELI-30999c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- C8, HOXC8 ELISA KIT

ELI-31002c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D13, HOXD13 ELISA KIT

ELI-31004c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D8, HOXD8 ELISA KIT

ELI-31005c 96 Tests
EUR 1113.6

Chicken Homeobox protein GBX- 2, GBX2 ELISA KIT

ELI-31259c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- B3, HOXB3 ELISA KIT

ELI-48719c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A5, HOXA5 ELISA KIT

ELI-37575c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D1, HOXD1 ELISA KIT

ELI-37581c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D9, HOXD9 ELISA KIT

ELI-48280c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D12, HOXD12 ELISA KIT

ELI-14318c 96 Tests
EUR 1113.6

Chicken LIM/homeobox protein Lhx3, LHX3 ELISA KIT

ELI-14467c 96 Tests
EUR 1113.6

Chicken Homeobox protein MSX- 2, MSX2 ELISA KIT

ELI-16603c 96 Tests
EUR 1113.6

Chicken Retinal homeobox protein Rx1, RX1 ELISA KIT

ELI-18616c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- B1, HOXB1 ELISA KIT

ELI-20229c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- D4, HOXD4 ELISA KIT

ELI-20235c 96 Tests
EUR 1113.6

Chicken Homeobox protein Hox- A3, HOXA3 ELISA KIT

ELI-20550c 96 Tests
EUR 1113.6

A protein fragment of Rv3194c positioned on mycobacterial cell flooring efficiently prevents adhesion of recombinant Mycobacterium smegmatis, and ensures a mannequin new anti-adhesive drug

Adhesins are virulence parts expressed on the surfaces of pathogenic micro organism that mediate pathogen-host interactions, a significant step all through the an an an infection course of. Correct proper right here, we present that the Mycobacterium tuberculosis protease Rv3194c capabilities not solely as an enzyme however as an adhesin. The heterologous Rv3194c protein was purified from Escherichia coli and was confirmed to bind to hyaluronic acid (HA).

The HA-binding web site was acknowledged as a 20 amino acid peptide between residues 91 and 110 (P91-110). Rv3194c sure to A549 alveolar basal epithelial cells and the interplay was abolished by the addition of hyaluronidase or P91-110. Experimental an an an infection in vitro revealed that Rv3194c participates all through the attachment of recombinant Mycobacterium smegmatis (Rv3194c/MS) to A549 cells, and P91-110 remedy of A549 cells largely inhibited the Rv3194c/MS-A549 cell interplay.

To produce in vivo proof, we constructed a reporter stress of M. smegmatis that expressed a by-product of the firefly luciferase that’s shifted to pink (FFlucRT) together with Rv3194c (Rv3194c + FFlucRT/MS) to contaminate mice and monitor the occasion of the illness. In mice, Rv3194c dramatically enhanced M. smegmatis persistence and induced lesions all through the lungs. Along with, remedy of intratracheal Rv3194c + FFlucRT/MS- contaminated mice with P91-110 considerably suppressed the expansion of Rv3194c + FFlucRT/MS in vivo and diminished pathological harm launched on by an an an infection of the lung with Rv3194c + FFlucRT/MS.

Taken collectively, these outcomes reveal that Rv3194c capabilities as an HA-binding adhesin and that P91-110 may have the potential for treating and stopping mycobacterial an an an infection.

Lysine acylation utilizing conjugating enzymes for site-specific modification and ubiquitination of recombinantproteins

Enzymes are extraordinarily environment friendly units for protein labelling attributable to their specificity and delicate response circumstances. Many protocols, nonetheless, are restricted to modifications at protein termini, rely on non-peptidic metabolites or require giant recognition domains. Correct proper right here we report a chemoenzymatic methodology, which we title lysine acylation utilizing conjugating enzymes (LACE), to site-specifically modify folded proteins at inside lysine residues. LACE relies upon upon a minimal genetically encoded tag (4 residues) acknowledged by the E2 small ubiquitin-like modifier-conjugating enzyme Ubc9, and peptide or protein thioesters.

Collectively, this technique obviates the necessity for E1 and E3 enzymes, enabling isopeptide formation with merely Ubc9 in a programmable methodology. We reveal the utility of LACE by the site-specific attachment of biochemical probes, one-pot dual-labelling collectively with sortase, and the conjugation of wild-type ubiquitin and ISG15 to recombinant proteins.

Responses of leaf gas alternate attributes, photosynthetic pigments and antioxidant enzymes in NaCl-stressed cotton (Gossypiumhirsutum L.) seedlings to exogenous glycine betaine and salicylic acid

Background: Utility of exogenous glycine betaine (GB) and exogenous salicylic acid (SA) mitigates the hostile outcomes of salinity. Foliar spraying with exogenous GB or SA alleviates salt stress in vegetation by rising leaf gas alternate and stimulating antioxidant enzyme train. The results of foliar utility of exogenous GB and SA on the physiology and biochemistry of cotton seedlings subjected to salt stress keep unclear.

Outcomes: Outcomes confirmed that salt stress of 150 mMNaCl significantly diminished leaf gas alternate and chlorophyll fluorescence and decreased photosynthetic pigment parts and leaf relative water content material materials.

Foliar spray concentrations of 5.Zero mM exogenous GB and 1.Zero mM exogenous SA promoted gas alternate and fluorescence in cotton seedlings, elevated parts of chlorophyll pigments, and stimulated the antioxidant enzyme train. The foliar spray moreover elevated leaf relative water content material materials and endogenous GB and SA content material materials in contrast with the salt-stressed solely administration.

Whatever the salt-induced improve in antioxidant enzyme content material materials, exogenous GB and SA in experimental concentrations significantly elevated the train of glutathione reductase, ascorbate peroxidase, superoxide dismutase, catalase and peroxidase, and decreased malondialdehyde content material materials beneath salt stress.

All through all experimental foliar spray GB and SA concentrations, the photochemical effectivity of photosystem II (FV/FM) reached a peak at a spotlight of 5.Zero mM GB. The net photosynthetic worth (Pn) and FV/FM had been positively correlated with chlorophyll a and chlorophyll b content material materials in response to foliar spraying of exogenous GB and SA beneath salt stress.

 

Conclusions: We concluded, from our outcomes, that concentrations of 5.Zero mM GB or 1.Zero mM SA are optimum selections for mitigating NaCl-induced hurt in cotton seedlings on account of they promote leaf photosynthesis, improve parts of photosynthetic pigments, and stimulate antioxidant enzyme train. Amongst, 5.Zero mM GB and 1.Zero mM SA, the easiest effectivity in enhancing endogenous GB and SA concentrations was obtained with the foliar utility of 1.Zero mM SA beneath salt stress.

 

Leave a Reply

Your email address will not be published. Required fields are marked *