Microscale Perfusion-based Cultivation for Pichia pastoris Clone

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redwoodbioscience

Microscale Perfusion-based Cultivation for Pichia pastoris Clone Screening Allows Accelerated and Optimized RecombinantProtein Manufacturing Processes

Pichia pastoris has emerged prior to now years as a promising host for recombinant protein and biopharmaceutical manufacturing. Contained in the institution of maximum cell density fed-batch biomanufacturing, screening half and early bioprocess progress (based mostly completely on microplates and shake flasks) nonetheless characterize a bottleneck attributable to high-cost and time-consuming procedures together with low experiment complexity.

Inside the current work, we implement a screening protocol developed for P. pastoris clone various in a multiplexed microfluidic machine with 15 μL cultivation chambers able to function in perfusion mode and monitor dissolved oxygen content material materials supplies all through the customized in a non-invasive means. The setup allowed us to determine carbon-limited circumstances and take into consideration stress responses to fully fully completely different enter variables. Outcomes from micro-scale perfusion cultures had been then in distinction with 1L fed-batch fermentation.

The best producer by way of titer and productiveness was shortly acknowledged after 12 hours from inoculation and the outcomes confirmed by lab-scale fermentation.

Furthermore, the physiological analyses of the strains beneath fully fully completely different circumstances steered how additional subtle experimental circumstances had been achievable regardless of the comparatively straightforward, straight-forward, and cost-effective experimental setup. Implementation and standardization of those micro-scale protocols may within the discount of the demand for lab-scale bioreactor cultivations thus accelerating the event of protein manufacturing processes. This textual content material is protected by copyright. All rights reserved.

redwoodbioscience
redwoodbioscience

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-10ug

QP5983-ec-10ug 10ug
EUR 391.2

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-200ug

QP5983-ec-200ug 200ug
EUR 1338

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-500ug

QP5983-ec-500ug 500ug
EUR 1816.8

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-50ug

QP5983-ec-50ug 50ug
EUR 522

Chicken Homeobox protein engrailed- 2, EN2 ELISA KIT

ELI-05953c 96 Tests
EUR 1113.6

Chicken Homeobox Protein Engrailed-2 (EN2) ELISA Kit

abx518615-96tests 96 tests
EUR 1093.2

Recombinant Engrailed Homeobox Protein 2 (EN2)

RPU55393-100ug 100ug
EUR 468.6

Recombinant Engrailed Homeobox Protein 2 (EN2)

RPU55393-1mg 1mg
EUR 2074.8

Recombinant Engrailed Homeobox Protein 2 (EN2)

RPU55393-50ug 50ug
EUR 376.2

Recombinant Engrailed Homeobox Protein 2 (EN2)

4-RPJ982Hu01
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  • 100 ug
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  • 5 mg
Description: Recombinant Human Engrailed Homeobox Protein 2 expressed in: E.coli

Homeobox Protein Engrailed-1 (EN1) Antibody

abx033297-400ul 400 ul
EUR 627.6

Homeobox Protein Engrailed-1 (EN1) Antibody

abx033297-80l 80 µl
EUR 343.2

Homeobox Protein Engrailed-1 (EN1) Antibody

20-abx215150
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  • 100 ug
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Homeobox Protein Engrailed-1 (EN1) Antibody

20-abx300471
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  • 100 ug
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Homeobox Protein Engrailed-1 (EN1) Antibody

abx033297-100g 100 µg
EUR 281.25

Homeobox Protein Engrailed-1 (EN1) Antibody

abx300471-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-1 (EN1) Antibody

abx300471-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-1 (EN1) Antibody

abx300471-50g 50 µg
EUR 250

Chicken Engrailed Homeobox Protein 2 ELISA kit

E01A91538 96T
EUR 700
Description: ELISA

Homeobox Protein Engrailed-1 (EN1) Antibody (HRP)

20-abx300472
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  • 100 ug
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Homeobox Protein Engrailed-1 (EN1) Antibody (HRP)

abx300472-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-1 (EN1) Antibody (HRP)

abx300472-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-1 (EN1) Antibody (HRP)

abx300472-50g 50 µg
EUR 250

Homeobox Protein Engrailed-1 (EN1) Antibody (FITC)

20-abx300473
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  • 100 ug
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Homeobox Protein Engrailed-1 (EN1) Antibody (FITC)

abx300473-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-1 (EN1) Antibody (FITC)

abx300473-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-1 (EN1) Antibody (FITC)

abx300473-50g 50 µg
EUR 250

Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin)

20-abx300474
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  • 100 ug
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Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin)

abx300474-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin)

abx300474-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin)

abx300474-50g 50 µg
EUR 250

Mouse Homeobox protein engrailed- 1, En1 ELISA KIT

ELI-27118m 96 Tests
EUR 1038

Human Homeobox protein engrailed- 1, EN1 ELISA KIT

ELI-48646h 96 Tests
EUR 988.8

Human Homeobox protein engrailed-1 (EN1) ELISA Kit

abx527211-96tests 96 tests
EUR 687.5

Mouse Homeobox protein engrailed-1 (EN1) ELISA Kit

abx527212-96tests 96 tests
EUR 687.5

EN1 (untagged)-Human engrailed homeobox 1 (EN1)

SC303016 10 µg Ask for price

EN1 (GFP-tagged) - Human engrailed homeobox 1 (EN1)

RG224659 10 µg Ask for price

EN1 (Myc-DDK-tagged)-Human engrailed homeobox 1 (EN1)

RC224659 10 µg Ask for price

Homeobox protein engrailed-2

AP79211 1mg
EUR 2640

Homeobox protein engrailed-2

AP83697 1mg
EUR 2640

Homeobox protein engrailed-2

AP84048 1mg
EUR 2640

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033410-400ul 400 ul
EUR 627.6

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033410-80l 80 µl
EUR 343.2

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033411-400ul 400 ul
EUR 627.6

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033411-80l 80 µl
EUR 343.2

Homeobox Protein Engrailed-2 (EN2) Antibody

20-abx302580
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  • 100 ug
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  • 200 ug
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Homeobox Protein Engrailed-2 (EN2) Antibody

abx302580-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-2 (EN2) Antibody

abx302580-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-2 (EN2) Antibody

abx302580-50g 50 µg
EUR 250

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033410-100g 100 µg
EUR 250

Homeobox Protein Engrailed-2 (EN2) Antibody

abx033411-100g 100 µg
EUR 281.25

Homeobox Protein Engrailed-2 (EN2) Antibody (HRP)

20-abx315705
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  • 100 ug
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Homeobox Protein Engrailed-2 (EN2) Antibody (HRP)

abx315705-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-2 (EN2) Antibody (HRP)

abx315705-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-2 (EN2) Antibody (HRP)

abx315705-50g 50 µg
EUR 250

Engrailed Homeobox Protein 2 (EN2) Antibody

20-abx172243
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  • 1 mg
  • 200 ug

Engrailed Homeobox Protein 2 (EN2) Antibody

20-abx176276
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  • 1 mg
  • 200 ug

Engrailed Homeobox Protein 2 (EN2) Antibody

20-abx176277
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Engrailed Homeobox Protein 2 (EN2) Antibody

abx172243-1ml 1 ml
EUR 787.5

Engrailed Homeobox Protein 2 (EN2) Antibody

abx176277-1096tests 10 × 96 tests
EUR 825

Engrailed Homeobox Protein 2 (EN2) Antibody

abx176277-596tests 5 × 96 tests
EUR 362.5

Engrailed Homeobox Protein 2 (EN2) Antibody

abx176277-96tests 96 tests
EUR 287.5

Homeobox Protein Engrailed-2 (EN2) Antibody (FITC)

20-abx315706
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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Homeobox Protein Engrailed-2 (EN2) Antibody (FITC)

abx315706-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-2 (EN2) Antibody (FITC)

abx315706-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-2 (EN2) Antibody (FITC)

abx315706-50g 50 µg
EUR 250

Human Engrailed Homeobox Protein 2 (EN2) Protein

20-abx652255
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  • 100 ug
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Human Engrailed Homeobox Protein 2 (EN2) Protein

abx652255-5mg 5 mg
EUR 362.5

Human Homeobox protein engrailed- 2, EN2 ELISA KIT

ELI-05951h 96 Tests
EUR 988.8

Mouse Homeobox protein engrailed- 2, En2 ELISA KIT

ELI-05952m 96 Tests
EUR 1038

Homeobox Protein Engrailed-2 (EN2) Antibody (Biotin)

20-abx315707
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  • 100 ug
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Homeobox Protein Engrailed-2 (EN2) Antibody (Biotin)

abx315707-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-2 (EN2) Antibody (Biotin)

abx315707-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-2 (EN2) Antibody (Biotin)

abx315707-50g 50 µg
EUR 250

Human Homeobox Protein Engrailed-2 (EN2) ELISA Kit

abx251286-96tests 96 tests
EUR 801.6

Mouse Homeobox Protein Engrailed-2 (EN2) ELISA Kit

abx518617-96tests 96 tests
EUR 801.6

Human Homeobox protein engrailed-2(EN2) ELISA kit

EKC41536-48T 48T
EUR 535.99

Human Homeobox protein engrailed-2(EN2) ELISA kit

EKC41536-5x96T 5x96T
EUR 3637.08

Human Homeobox protein engrailed-2(EN2) ELISA kit

EKC41536-96T 96T
EUR 765.7

Human Homeobox protein engrailed-2(EN2) Elisa Kit

EK712182 96 Wells
EUR 0.24

Human Homeobox protein engrailed-2(EN2) ELISA kit

CSB-EL007660HU-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Homeobox protein engrailed-2 (EN2) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Homeobox protein engrailed-2(EN2) ELISA kit

1-CSB-EL007660HU
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Homeobox protein engrailed-2(EN2) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Homeobox protein engrailed-2 (EN2) ELISA Kit

RK08809 96T
EUR 280

Human Homeobox protein engrailed-2 (EN2) ELISA Kit

RK01311 96 Tests
EUR 280

Engrailed Homeobox 2 Protein (OVA)

20-abx165529
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human EN2(Homeobox protein engrailed-2) ELISA Kit

EH1964 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human EN2/ Homeobox protein engrailed-2 ELISA Kit

E0787Hu 1 Kit
EUR 515

Human EN2(Homeobox protein engrailed-2) ELISA Kit

EKF57407-48T 48T
EUR 396.9

Human EN2(Homeobox protein engrailed-2) ELISA Kit

EKF57407-5x96T 5x96T
EUR 2693.25

Human EN2(Homeobox protein engrailed-2) ELISA Kit

EKF57407-96T 96T
EUR 567

Engrailed Homeobox Protein 2 (EN2) Antibody Pair

20-abx370677
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  • 10 × 96 tests
  • 5 × 96 tests

Engrailed Homeobox Protein 2 (EN2) Antibody Pair

abx370677-100g 100 µg
EUR 2362.5

Engrailed Homeobox Protein 2 (EN2) Antibody Pair

abx370677-50g 50 µg
EUR 1462.5

Human Homeobox Protein engrailed-2, EN2 GENLISA ELISA

KBH5427 1 x 96 wells
EUR 286

OVA Conjugated Engrailed Homeobox Protein 2 (EN2)

CPJ982Hu21 10ug
EUR 140

OVA conjugated Engrailed Homeobox Protein 2 (EN2)

4-CPJ982Hu21
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  • 100 ug
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  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Engrailed Homeobox Protein 2 expressed in: chemical synthesis

OVA Conjugated Engrailed Homeobox Protein 2 (EN2)

RPU50907-100ug 100ug
EUR 431.2

OVA Conjugated Engrailed Homeobox Protein 2 (EN2)

RPU50907-1mg 1mg
EUR 1911

OVA Conjugated Engrailed Homeobox Protein 2 (EN2)

RPU50907-50ug 50ug
EUR 336

Human Engrailed Homeobox Protein 2 (EN2) CLIA Kit

20-abx495807
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

20-abx151405
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Engrailed Homeobox Protein 2 (EN2)ELISA Kit

201-12-2671 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

abx571248-96tests 96 tests
EUR 990

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

DLR-EN2-Hu 96T
EUR 463
Description: tissue homogenates or other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

DLR-EN2-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates or other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

DLR-EN2-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates or other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKN45006-48T 48T
EUR 378.7

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKN45006-5x96T 5x96T
EUR 2569.75

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKN45006-96T 96T
EUR 541

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKL57008-5x96T 5x96T
EUR 3793.35

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKL57008-96T 96T
EUR 798.6

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EK13816 96Т
EUR 768

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKU03895-48T 48T
EUR 555.66

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKU03895-5x96T 5x96T
EUR 3770.55

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

EKU03895-96T 96T
EUR 793.8

Human EN2(Engrailed Homeobox Protein 2) ELISA Kit

ELK3841-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EN2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human EN2(Engrailed Homeobox Protein 2) ELISA Kit

ELK3841-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EN2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

DL-EN2-Hu 96T
EUR 441
Description: tissue homogenates or other biological fluids.

Human Engrailed Homeobox Protein 2(EN2)ELISA Kit

QY-E04943 96T
EUR 433.2

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RDR-EN2-Hu-48T 48T
EUR 475.34
Description: tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RDR-EN2-Hu-48Tests 48 Tests
EUR 652.8

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RDR-EN2-Hu-96T 96T
EUR 679.04
Description: tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RDR-EN2-Hu-96Tests 96 Tests
EUR 907.2

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RD-EN2-Hu-48T 48T
EUR 452.7
Description: tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RD-EN2-Hu-48Tests 48 Tests
EUR 625.2

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RD-EN2-Hu-96T 96T
EUR 646.7
Description: tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

RD-EN2-Hu-96Tests 96 Tests
EUR 867.6

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

SEJ982Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.

Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

4-SEJ982Hu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Engrailed Homeobox Protein 2 (EN2)ELISA Kit

YLA3312HU-48T 48T Ask for price

Human Engrailed Homeobox Protein 2 (EN2)ELISA Kit

YLA3312HU-96T 96T Ask for price

Lenti ORF clone of Human engrailed homeobox 1 (EN1), mGFP tagged

RC224659L4 10 µg Ask for price

Engrailed Homeobox Protein 2 (EN2) Polyclonal Antibody

CAU31880-100ul 100ul
EUR 258.2

Engrailed Homeobox Protein 2 (EN2) Polyclonal Antibody

CAU31880-200ul 200ul
EUR 322.6

Human Engrailed Homeobox Protein 2, EN2 GENLISA ELISA

KBH2670 1 x 96 wells
EUR 286

ELISA kit for Human EN2 (Engrailed Homeobox Protein 2)

ELK3841 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Engrailed Homeobox Protein 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Human Homeobox protein engrailed-2 ELISA Kit

EK4019 96 tests
EUR 599

Lenti ORF particles, EN1 (mGFP-tagged) - Human engrailed homeobox 1 (EN1), 200ul, >10^7 TU/mL

RC224659L4V 200 µl Ask for price

Rat Engrailed Homeobox Protein 2 ELISA kit

E02E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Engrailed Homeobox Protein 2 ELISA kit

E02E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Engrailed Homeobox Protein 2 ELISA kit

E02E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Engrailed Homeobox Protein 2 ELISA kit

E07E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Engrailed Homeobox Protein 2 ELISA kit

E07E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Engrailed Homeobox Protein 2 ELISA kit

E07E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Engrailed Homeobox Protein 2 ELISA kit

E08E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Engrailed Homeobox Protein 2 ELISA kit

E08E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Engrailed Homeobox Protein 2 ELISA kit

E08E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Engrailed Homeobox Protein 2 ELISA kit

E01A13042 96T
EUR 700
Description: ELISA

Goat Engrailed Homeobox Protein 2 ELISA kit

E01A47951 96T
EUR 700
Description: ELISA

Goat Engrailed Homeobox Protein 2 ELISA kit

E06E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Engrailed Homeobox Protein 2 ELISA kit

E06E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Engrailed Homeobox Protein 2 ELISA kit

E06E0151-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Lenti ORF clone of Human engrailed homeobox 1 (EN1), Myc-DDK-tagged

RC224659L3 10 µg Ask for price

Mouse Engrailed Homeobox Protein 2 ELISA kit

E01A21782 96T
EUR 700
Description: ELISA

Human Engrailed Homeobox Protein 2 ELISA kit

E01A4278 96T
EUR 700
Description: ELISA

Mouse Engrailed Homeobox Protein 2 ELISA kit

E03E0151-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Engrailed Homeobox Protein 2 ELISA kit

E03E0151-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

A protein fragment of Rv3194c positioned on mycobacterial cell flooring efficiently prevents adhesion of recombinant Mycobacterium smegmatis, and ensures a mannequin new anti-adhesive drug

Adhesins are virulence parts expressed on the surfaces of pathogenic micro organism that mediate pathogen-host interactions, a significant step all through the an an an infection course of. Correct proper right here, we present that the Mycobacterium tuberculosis protease Rv3194c capabilities not solely as an enzyme however as an adhesin. The heterologous Rv3194c protein was purified from Escherichia coli and was confirmed to bind to hyaluronic acid (HA).

The HA-binding web site was acknowledged as a 20 amino acid peptide between residues 91 and 110 (P91-110). Rv3194c sure to A549 alveolar basal epithelial cells and the interplay was abolished by the addition of hyaluronidase or P91-110. Experimental an an an infection in vitro revealed that Rv3194c participates all through the attachment of recombinant Mycobacterium smegmatis (Rv3194c/MS) to A549 cells, and P91-110 remedy of A549 cells largely inhibited the Rv3194c/MS-A549 cell interplay.

To produce in vivo proof, we constructed a reporter stress of M. smegmatis that expressed a by-product of the firefly luciferase that’s shifted to pink (FFlucRT) together with Rv3194c (Rv3194c + FFlucRT/MS) to contaminate mice and monitor the occasion of the illness. In mice, Rv3194c dramatically enhanced M. smegmatis persistence and induced lesions all through the lungs. Along with, remedy of intratracheal Rv3194c + FFlucRT/MS- contaminated mice with P91-110 considerably suppressed the expansion of Rv3194c + FFlucRT/MS in vivo and diminished pathological harm launched on by an an an infection of the lung with Rv3194c + FFlucRT/MS.

Taken collectively, these outcomes reveal that Rv3194c capabilities as an HA-binding adhesin and that P91-110 may have the potential for treating and stopping mycobacterial an an an infection.

Lysine acylation utilizing conjugating enzymes for site-specific modification and ubiquitination of recombinantproteins

Enzymes are extraordinarily environment friendly units for protein labelling attributable to their specificity and delicate response circumstances. Many protocols, nonetheless, are restricted to modifications at protein termini, rely on non-peptidic metabolites or require giant recognition domains. Correct proper right here we report a chemoenzymatic methodology, which we title lysine acylation utilizing conjugating enzymes (LACE), to site-specifically modify folded proteins at inside lysine residues. LACE relies upon upon a minimal genetically encoded tag (4 residues) acknowledged by the E2 small ubiquitin-like modifier-conjugating enzyme Ubc9, and peptide or protein thioesters.

Collectively, this technique obviates the necessity for E1 and E3 enzymes, enabling isopeptide formation with merely Ubc9 in a programmable methodology. We reveal the utility of LACE by the site-specific attachment of biochemical probes, one-pot dual-labelling collectively with sortase, and the conjugation of wild-type ubiquitin and ISG15 to recombinant proteins.

Responses of leaf gas alternate attributes, photosynthetic pigments and antioxidant enzymes in NaCl-stressed cotton (Gossypiumhirsutum L.) seedlings to exogenous glycine betaine and salicylic acid

Background: Utility of exogenous glycine betaine (GB) and exogenous salicylic acid (SA) mitigates the hostile outcomes of salinity. Foliar spraying with exogenous GB or SA alleviates salt stress in vegetation by rising leaf gas alternate and stimulating antioxidant enzyme train. The results of foliar utility of exogenous GB and SA on the physiology and biochemistry of cotton seedlings subjected to salt stress keep unclear.

Outcomes: Outcomes confirmed that salt stress of 150 mMNaCl significantly diminished leaf gas alternate and chlorophyll fluorescence and decreased photosynthetic pigment parts and leaf relative water content material materials.

Foliar spray concentrations of 5.Zero mM exogenous GB and 1.Zero mM exogenous SA promoted gas alternate and fluorescence in cotton seedlings, elevated parts of chlorophyll pigments, and stimulated the antioxidant enzyme train. The foliar spray moreover elevated leaf relative water content material materials and endogenous GB and SA content material materials in contrast with the salt-stressed solely administration.

Whatever the salt-induced improve in antioxidant enzyme content material materials, exogenous GB and SA in experimental concentrations significantly elevated the train of glutathione reductase, ascorbate peroxidase, superoxide dismutase, catalase and peroxidase, and decreased malondialdehyde content material materials beneath salt stress.

All through all experimental foliar spray GB and SA concentrations, the photochemical effectivity of photosystem II (FV/FM) reached a peak at a spotlight of 5.Zero mM GB. The net photosynthetic worth (Pn) and FV/FM had been positively correlated with chlorophyll a and chlorophyll b content material materials in response to foliar spraying of exogenous GB and SA beneath salt stress.

 

Conclusions: We concluded, from our outcomes, that concentrations of 5.Zero mM GB or 1.Zero mM SA are optimum selections for mitigating NaCl-induced hurt in cotton seedlings on account of they promote leaf photosynthesis, improve parts of photosynthetic pigments, and stimulate antioxidant enzyme train. Amongst, 5.Zero mM GB and 1.Zero mM SA, the easiest effectivity in enhancing endogenous GB and SA concentrations was obtained with the foliar utility of 1.Zero mM SA beneath salt stress.

 

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