Immunization with a RecombinantProtein of Trichinella britovi

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redwoodbioscience

Institution of a two-step purification scheme for tag-free recombinant Taiwan native norovirus P and VP1 proteins

The protruding (P) area of the necessary factor capsid protein VP1 of norovirus (NoV) is the necessary concern for immune recognition and host receptor binding. The heterologous P protein expressed by Pichia pastoris self-assembles into P particles. Nonetheless, tag-free NoV protein purification schemes have hardly been reported due to low isoelectric diploma of NoV proteins, which results in terribly aggressive binding between the intention protein and yeast host cell proteins at alkaline pH.

On this evaluation, a two-step purification scheme based mostly completely on flooring histidines and the charge on the NoV GII.Four stress P protein was developed. Utilizing HisTrap and ion commerce chromatography, the P protein was straight purified, with a restoration of 28.1% and purity of 82.1%. Equally, the NoV capsid protein VP1 was furthermore purified utilizing HisTrap and gel filtration chromatography based mostly completely on native flooring histidines and self-assembly potential, with 20% restoration and over 90% purity.

Dynamic delicate scattering and transmission electron microscopy analyses of the purified NoV P revealed that most of those small P particles had been triangle-, square- and ring-shaped, with a diameter of roughly 14 nm, and that the purified NoV VP1 self-assembles into particles with a diameter of roughly 47 nm. Each the purified NoV P and VP1 particles retained human histo-blood group antigen-binding potential, as evidenced by a saliva-binding assay.

redwoodbioscience
redwoodbioscience

Glypican 6 (GPC6) Antibody

20-abx213390
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Human glypican 6 (GPC6) ELISA kit

CSB-EL009708HU-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human glypican 6 (GPC6) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human glypican 6 (GPC6) ELISA kit

1-CSB-EL009708HU
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human glypican 6 (GPC6) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Glypican 6(GPC6)ELISA Kit

QY-E02274 96T
EUR 433.2

Individual Reaction Mix 6

G065-6 200 reactions
EUR 200.4

Recombinant Human Glypican-6 Protein, His, Yeast-100ug

QP9160-ye-100ug 100ug
EUR 576

Recombinant Human Glypican-6 Protein, His, Yeast-10ug

QP9160-ye-10ug 10ug
EUR 283.2

Recombinant Human Glypican-6 Protein, His, Yeast-1mg

QP9160-ye-1mg 1mg
EUR 2262

Recombinant Human Glypican-6 Protein, His, Yeast-200ug

QP9160-ye-200ug 200ug
EUR 892.8

Recombinant Human Glypican-6 Protein, His, Yeast-500ug

QP9160-ye-500ug 500ug
EUR 1447.2

Recombinant Human Glypican-6 Protein, His, Yeast-50ug

QP9160-ye-50ug 50ug
EUR 358.8

Random Nanofibers 6 Well Plate

3D00006-6 700 nm-PCLs
EUR 111.6

Aligned Nanofibers 6 Well Plate

3D00012-6 700 nm-PCLs
EUR 116.4

pLenti-CLDN1 shRNA-6 Plasmid

PVTBAV04867-6 2 ug
EUR 427.2

Tissue Culture Plate, 6 Well

TCP20-6 1 UNIT
EUR 64.18

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 6 vials)

M1040-6
EUR 1560

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 6 vials)

M1041-6
EUR 1886.4

ExoStd? Lyophilized Exosome Standard (30 µg, Human Serum, 6 vials)

M1042-6
EUR 1560

ExoStd? Lyophilized Exosome Standard (100 µg, Human Serum, 6 vials)

M1043-6
EUR 1886.4

ExoStd? Lyophilized Exosome Standard (30 µg, Human Urine, 6 vials)

M1044-6
EUR 1546.8

ExoStd? Lyophilized Exosome Standard (100 µg, Human Urine, 6 vials)

M1045-6
EUR 1886.4

ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 6 vials)

M1046-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 6 vials)

M1047-6
EUR 1945.2

Aligned Nanofibers 6 Well Plate Inserts

3D00016-6 700 nm-PCLs
EUR 117.6

Pesticide Mix 6 Containing 15 Compounds in Acetonitrile

SPXPR-6 1ML
EUR 182.4

ExoStd? Lyophilized Exosome Standard (30 µg, U87 MG, 6 vials)

M1054-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, U87 MG, 6 vials)

M1055-6
EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, COLO1 cell line, 6 vials)

M1048-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, COLO1 cell line, 6 vials)

M1049-6
EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, MM1 cell line, 6 vials)

M1050-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, MM1 cell line, 6 vials)

M1051-6
EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, BLCL21 cell line, 6 vials)

M1052-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, BLCL21 cell line, 6 vials)

M1053-6
EUR 1886.4

ExoStd? Lyophilized Exosome Standard (30 µg, HCT116 cell line, 6 vials)

M1058-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, HCT116 cell line, 6 vials)

M1059-6
EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, PC3 cell line, 6 vials)

M1060-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, PC3 cell line, 6 vials)

M1061-6
EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, DAUD1 cell line, 6 vials)

M1064-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, DAUD1 cell line, 6 vials)

M1065-6
EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, A549 cell line, 6 vials)

M1066-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, A549 cell line, 6 vials)

M1067-6
EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, B16F10 cell line, 6 vials)

M1070-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (1090 µg, B16F10 cell line, 6 vials)

M1071-6
EUR 1939.2

Human GPC6 (Glypican- 6) ELISA Kit (CUSTOM ELISA KIT)

ELI-48039h 96 Tests
EUR 988.8

ExoStd? Lyophilized Exosome Standard (30 µg, BPH-1 cell line, 6 vials)

M1062-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, BPH-1 cell line, 6 vials)

M1063-6
EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, K-562 cell line, 6 vials)

M1068-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, K-562 cell line, 6 vials)

M1069-6
EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, SK-N-SH cell line, 6 vials)

M1056-6
EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, SK-N-SH cell line, 6 vials)

M1057-6
EUR 1939.2

Recombinant Human HGF Protein

PROTP14210-6 10ug
EUR 380.4
Description: HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues.*Manufactured using (BTI-Tn-5B1-4) cells under license from Boyce Thompson Institute for Plant Research, Inc.

Recombinant Human Myostatin Protein

PROTO14793-6 10ug
EUR 380.4
Description: Myostatin is a TGF-β family member that acts as an inhibitor of skeletal muscle growth. This muscle-specific cytokine interacts with Activin type I and type II receptors, and suppresses myoblast proliferation by arresting cell-cycle in the G1 phase. Suppression of myostatin activity facilitates muscle formation and may be useful in reducing and/or preventing adiposity and type-2 diabetes. Myostatin activity can be blocked by the Activin-binding protein Follistatin, and by the propeptide of Myostatin. Recombinant Human myostatin is a 25.0 kDa protein consisting of two identical 109 amino acid polypeptides linked by a single disulfide bond.

Human Glypican 1 (GPC1) Protein

20-abx066889
  • EUR 627.60
  • EUR 292.80
  • EUR 1796.40
  • EUR 727.20
  • EUR 460.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Glypican 3 (GPC3) Protein

20-abx066892
  • EUR 744.00
  • EUR 326.40
  • EUR 2230.80
  • EUR 878.40
  • EUR 543.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Glypican 4 (GPC4) Protein

20-abx066893
  • EUR 895.20
  • EUR 360.00
  • EUR 2798.40
  • EUR 1062.00
  • EUR 627.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Glypican 3 (GPC3) Protein

abx060155-100ug 100 ug
EUR 1362

Human Glypican 3 (GPC3) Protein

abx060263-1mg 1 mg
EUR 2532

Human Glypican-1 (GPC1) Protein

abx060282-100ug 100 ug
EUR 944.4

Human Glypican 2 (GPC2) Protein

20-abx653616
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Recombinant Human Glypican 3 Protein

RP00138 10 μg
EUR 186

Recombinant Human Glypican 1 Protein

RP00297 20 μg
EUR 231.6

Acrp30 Human, Adiponectin Human Recombinant Protein, HEK

PROTQ15848-6 Regular: 10ug
EUR 380.4
Description: The Acrp30 Human Recombinant produced in HEK293 cells is fused with FLAG tag.

Canadian Pesticide Mix 6 Containing 11 Compounds

CAN-CAN-6 1ML
EUR 84.36

Recombinant Human IL-7 Protein

PROTP13232-6 10ug
EUR 380.4
Description: IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant human IL-7 is a 17.4 kDa protein containing 153 amino acid residues.

Recombinant Human PlGF-3 Protein

PROTP49763-6 25ug
EUR 380.4
Description: PlGF-3 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PlGF-3 is expressed exclusively in the placenta. It signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration. PlGF-3 lacks heparin binding affinity. Recombinant human PlGF-3 is a 45.7 kDa disulfide-linked homodimeric protein of two 203 amino acid polypeptide chains.

Recombinant Human GM-CSF Protein

PROTP04141-6 20ug
EUR 380.4
Description: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

BCL2 Human, B-Cell Lymphoma Protein 2 Alpha Human Recombinant Protein, His Tag

PROTP10415-6 Regular: 20ug
EUR 380.4
Description: BCL2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-211 and having a molecular mass of 25.4 kDa. The BCL2 is fused to a 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Human IL-1 beta Recombinant Protein

R00101-6 5ug/vial
EUR 310.8
Description: IL-1 beta (IL-1β) is a member of the interleukin 1 family of cytokines. The IL-1 beta cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. Human IL-1 beta Recombinant Protein is purified interleukin-1 beta cytokine produced in yeast.

22 gauge small hub needles (Pack of 6)

MLCP-HN22-6 6 X 22 GUAGE
EUR 54
Description: 22 gauge small hub needles (Pack of 6)

VEGF Human, Vascular Endothelial Growth Factor Human Recombinant Protein, His

PROTP15692-6 Regular: 20ug
EUR 380.4
Description: Vascular Endothelial Growth Factor Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain (aa 207-371) containing a total of 185 amino acids and having a molecular mass of 21.3 kDa (corresponding to Isoform L-VEGF165 UniProt acc#P15692-11). ;The VEGF is fused to a 20 a.a His-tag at N-terminus and is purified by proprietary chromatographic techniques.

Recombinant Human FGF-basic (154 a.a.) Protein

PROTP09038-6 50ug
EUR 380.4
Description: FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.

Recombinant Human Oncostatin M (227 a.a.) Protein

PROTP13725-6 10ug
EUR 380.4
Description: Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi's sarcoma cells, OSH can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi's sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. Recombinant human Oncostatin M is a 25.7 kDa protein, containing 227 amino acid residues.

Recombinant Human SDF-1 Beta (CXCL12) Protein

PROTP48061-6 10ug
EUR 380.4
Description: SDF-1α and β are stromal derived CXC chemokines, and signal through the CXCR4 receptor. SDF-1α and β chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor. Recombinant human SDF-1β is an 8.5 kDa protein containing 72 amino acid residues.

AFP (Alpha fetoprotein) ELISA test

6 96T/Box Ask for price
Description: ELISA based test for quantitative detection of AFP (Alpha fetoprotein)

IC Multi Elem (6) Anion Std 10ppm in H2O

ICA-10PPM-6 100ML
EUR 269.04

Recombinant Human TGF-Beta 3 (E.coli derived) Protein

PROTP10600-6 10ug
EUR 380.4
Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. The physiological role of TGF-β3 is still unknown but its expression pattern suggests a role in the regulation of certain development processes. Recombinant human TGF-β3 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

SNCA Alpha Synuclein 96-140 Human Recombinant Protein

PROTP37840-6 Regular: 50ug
EUR 380.4
Description: A-Synuclein 96-140 Human Recombinant which is a deletion mutant of the a-synuclein amino acids 96-140, produced in E.Coli is a single, non-glycosylated polypeptide chain of 46 amino acids having a molecular mass of 5.2kDa, with an additional Met attached at the N-terminus. The Recombinant Human a-Synuclein 96-140 is purified by proprietary chromatographic techniques.

GLP-2 Human Glucagon Like Peptide-2 protein

PROTP01275-6 Regular: 5mg
EUR 380.4
Description: Glucagon Like Peptide-2 contains 34 amino acids having a molecular mass of 3922.38 Dalton.

Mouse GPC6 (Glypican- 6) ELISA Kit (CUSTOM ELISA KIT)

ELI-27268m 96 Tests
EUR 1038

Glypican 1 Recombinant Protein

91-578 0.05 mg
EUR 588.3
Description: Glypican-1 is a cell membrane protein and belongs to the glypican family. The protein may act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. It is required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.

Glypican 4 (GPC4) Protein

20-abx262759
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Glypican 3 Recombinant Protein

96-360 0.1 mg
EUR 682.8
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein

96-803 0.1 mg
EUR 852.9
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein

96-804 0.1 mg
EUR 651.3
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein

97-001 0.1 mg
EUR 682.8
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein

97-002 0.1 mg
EUR 651.3
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

FLT1 Human, Vascular Endothelial Growth Factor receptor-1 Human Recombinant Protein, His Tag

PROTP17948-6 Regular: 10ug
EUR 380.4
Description: FLT1 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 298 amino acids fragment (31-328) corresponding to the IgG like domains 1-3 from the mature soluble FLT1 protein, having a total molecular mass of 43kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. ;The FLT1 His is purified by proprietary chromatographic techniques.

GPC3 Glypican-3 Human Recombinant Protein

PROTP51654 Regular: 10ug
EUR 380.4
Description: GPC3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 544 amino acids (25-559a.a.) and having a molecular mass of 61.8kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). GPC3 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.

GPC4 Glypican-4 Human Recombinant Protein

PROTO75487 Regular: 10ug
EUR 380.4
Description: GPC4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids (401-529a.a) and having a molecular mass of 18kDa.GPC4 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Recombinant Human TGF-Beta 1 (HEK 293 derived) Protein

PROTP01137-6 10ug
EUR 380.4
Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.

MicroCrystal Mount Assortment, box of 20; Model #6, on 18mm rods

M4-L18SP-6 20 MOUNTS
EUR 125
Description: MicroCrystal Mount Assortment, box of 20; Model #6, on 18mm rods

Glypican 2 / GPC2 Recombinant Protein

11-254 0.1 mg
EUR 714.3
Description: Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

Glypican 1 / GPC1 Recombinant Protein

11-321 0.1 mg
EUR 714.3
Description: Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Glypican 2 / GPC2 Recombinant Protein

11-322 0.1 mg
EUR 714.3
Description: Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

Glypican 1 / GPC1 Recombinant Protein

11-327 0.1 mg
EUR 714.3
Description: Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Glypican 1 / GPC1 Recombinant Protein

11-328 0.1 mg
EUR 714.3
Description: Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Glypican 2 / GPC2 Recombinant Protein

11-329 0.1 mg
EUR 714.3
Description: Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

Glypican 3 / GPC3 Recombinant Protein

11-400 0.1 mg
EUR 682.8
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Mouse Glypican 1 (GPC1) Protein

20-abx066890
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Glypican 1 (GPC1) Protein

20-abx066891
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Glypican 4 (GPC4) Protein

20-abx066894
  • EUR 844.80
  • EUR 343.20
  • EUR 2631.60
  • EUR 1011.60
  • EUR 610.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Glypican 4 (GPC4) Protein

20-abx066895
  • EUR 910.80
  • EUR 360.00
  • EUR 2865.60
  • EUR 1095.60
  • EUR 644.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Glypican 3 (GPC3) Protein

20-abx167236
  • EUR 794.40
  • EUR 326.40
  • EUR 2431.20
  • EUR 944.40
  • EUR 577.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Glypican 5 (GPC5) Protein

20-abx650459
  • EUR 661.20
  • EUR 292.80
  • EUR 1914.00
  • EUR 777.60
  • EUR 493.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Glypican 3 (GPC3) Protein

20-abx653617
  • EUR 777.60
  • EUR 326.40
  • EUR 2397.60
  • EUR 927.60
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Glypican-4, 511 a.a. Protein

20-abx262795
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

SPA-Cys Staphylococcal Protein-A Cys Long Form Recombinant Protein

PROTP38507-6 Regular: 100mg
EUR 501.6
Description: SPA-Cys long Recombinant produced in E.Coli is a single non-glycosylated polypeptide chain with a Cys on C-terminus. SPA-Cys is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 423 amino acids and having a molecular mass of 46.7kDa containing little or no carbohydrate.

Ovine CXCL10 Recombinant Protein

R00278-6 5ug/vial
EUR 310.8
Description: The ELR-negative CXC chemokine CXCL10 (IP-10) has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. Ovine CXCL10 Recombinant Protein is purified CXCL10 (IP-10) produced in yeast.

Rat CCL4 Recombinant Protein

R00703-6 5ug/vial
EUR 310.8
Description: The small chemotactic cytokine CCL4 (MIP-1 beta) is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is involved in several inflammatory and autoimmune diseases including viral infection such as HIV-1/AIDS. Rat CCL4 Recombinant Protein is purified chemokine ligand 4 (CCL4, MIP-1 beta) produced in yeast.

Swine CXCL9 Recombinant Protein

R01397-6 5ug/vial
EUR 310.8
Description: CXCL9 (MIG) is a T-cell chemoattractant. Induced by IFN-gamma (IFN-γ), the ELR-negative chemokine CXCL9 (MIG) elicits its effects by binding to the cell surface chemokine receptor CXCR3. Swine CXCL9 Recombinant Protein is purified CXCL9 (MIG) produced in yeast.

Immunization with a RecombinantProtein of Trichinella britovi 14-3-Three Triggers an Immune Response nonetheless No Safety in Mice

14-3-Three proteins are current in all eukaryotic organisms and are ubiquitously expressed in a broad fluctuate of tissues and mobile compartments. They’re regulatory adapter proteins that play key roles in quite a lot of signaling pathways, and have been proposed as relevant targets for the administration and detection of sure parasites.

  • Trichinella britovi is a widely-distributed parasitic nematode, transmitted by means of ingestion of meat merchandise containing invasive larvae. The current evaluation describes the cloning and expression of Tb14-3-3, and investigates the immunological and defending potential of the recombinant protein. Immunization of mice with rTb14-3-Three triggered an IgG response, and important variations, all through the profiles of secreted cytokines noticed in vitro, between experimental teams.
  • Nonetheless, neither specific antibodies, nor elevated secretion of IFNγ, IL-4, and IL-10 cytokines, conferred elevated safety within the route of an an an infection. No low value in larval burden was noticed all via restoration at 48 dpi. Moreover, rTb14-3-Three was not acknowledged by sera from the contaminated administration mice, aside from one, suggesting some mismatch between native and recombinant Tb14-3-Three antigenic websites. Attributable to this actuality, before 14-3-Three might probably be thought of a possible software program program for Trichinella detection and vaccination, additional analysis concerning its intention proteins, and precise specific perform, is required.

Inhibition of E. coli host RNA Polymerase Permits Setting nice Extracellular RecombinantProtein Manufacturing by Enhancing Outer Membrane Leakiness

Recombinant proteins in Escherichia coli are often expressed contained inside the cell. With the rising curiosity in common cultivation, secretion of product to the medium is solely not solely a income nonetheless a necessity in future bioprocessing. On this evaluation, we present that induced decoupling of progress and heterologous gene expression all through the E. coli X-press stress, a stress derived from BL21(DE3), facilitates extracellular recombinant protein manufacturing. We investigated the affect of the technique parameters temperature and specific glucose consumption value (qS ) on progress, productiveness, lysis and leakiness, to go looking the parameter area permitting extracellular protein manufacturing.

  • Two mannequin proteins had been used, Protein A and a VHH single-domain antibody, and effectivity was in contrast with the economic common stress BL21(DE3). We present that inducible progress repression all through the X-press stress vastly mitigates the affect of metabolic burden beneath utterly utterly completely different course of situations.
  • Moreover, temperature and qS had been used to manage productiveness and leakiness. Contained in the X-press stress, extracellular Protein A and VHH titer reached as loads as 349 mg/g and 19.6 mg/g, respectively, comprising as loads as 90% of the overall soluble product, whereas preserving cell lysis at a minimal. Our findings reveal that the X-press stress constitutes a useful host for extracellular manufacturing of recombinant proteinwith E. coli. This textual content material is protected by copyright. All rights reserved.

Rubisco lysine acetylation occurs at very low stoichiometry in mature Arabidopsis leaves: implications for regulation of enzyme carry out

Quite a lot of analysis have confirmed ribulose-1,5-bisphosphate carboxylase/oxygenase (E.C. 4.1.1.39; Rubisco) to be subject to Lys-acetylation at quite a few residues; nonetheless, opposing experiences exist regarding the natural significance of these post-translational modifications. One facet of the Lys-acetylation that has not been addressed in vegetation sometimes, or with Rubisco notably, is the stoichiometry at which these Lys-acetylation events occur.

As a way to find out which Lys-acetylation web sites on Arabidopsis Rubisco is maybe of regulatory significance to its catalytic carry out inside the Calvin-Benson cycle, we purified Rubisco from leaves in every the day and night-time and carried out unbiased mass-spectrometry based totally methods to search out out the stoichiometry of Rubisco Lys-acetylation events. The outcomes level out that Rubisco is acetylated at most Lys residues, nevertheless each acetylation event occurs at very low stoichiometry.

Furthermore, in vitro therapies that elevated the extent of Lys-acetylation on purified Rubisco had no affect on Rubisco maximal train. Resulting from this truth, we’re unable to substantiate that Lys-acetylation at low stoichiometries is often a regulatory mechanism controlling Rubisco maximal train. The outcomes highlight the need for extra use of stoichiometry measurements when determining the natural significance of reversible PTMs like acetylation.

Selective Inhibition of Zophobasmorio luciferase-like enzyme luminescence by diclofenac and potential suitability for gentle off biosensing

The buildup of toxic carboxylic compounds might set off excessive outcomes on the environment and dwelling organisms. A luciferase-like enzyme, beforehand cloned from the Malpighian tubules of the non-luminescent Zophobasmorio mealworm, reveals thioesterification train with quite a lot of carboxylic substrates, and produces weak purple luminescence inside the presence of ATP and firefly D-luciferin, a xenobiotic for this organism.

To larger study the carry out of this enzyme in carboxylic xenobiotic cleaning, we analyzed the inhibitory affect of assorted xenobiotic carboxylic acids on the luminescence train of this enzyme, along with environmental air pollution and pharmaceutical compounds.

Noteworthy, the anti-inflammatory drug diclofenac severely inhibited this luciferase-like enzyme (IC50 ~20 μM), when as compared with completely different beetle luciferases. Associated outcomes had been obtained with its brighter I327S mutant. Kinetic analysis of diclofenac’s affect on luminescence train indicated mixed-type inhibition for every ATP and D-luciferin.

Modeling analysis confirmed 5 potential binding web sites for diclofenac, along with the CoA binding website, which confirmed one in all many highest binding fastened. Taken collectively, these outcomes enhance the chance of using this luciferase-like enzyme for the occasion of novel total cell luminescent biosensors for diclofenac and comparable treatment.

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